Kinetic and Structural Studies of Hydroxyproline 2-Epimerase
نویسندگان
چکیده
منابع مشابه
Kinetic and structural studies of hydroxyproline 2-epimerase.
Hydroxyproline Z-epimerase, purified to homogeneity from hydroxyproline-induced cells of Pseudomonas pufida, behaved like a single peptide chain of approximately 64,000 molecular weight, based both on ultracentrifugal data and on a study of tryptic peptides and amino acid composition. Several methods indicated a total of 12 reduced cysteines per mole of native enzyme. Of these only six reacted ...
متن کاملMolecular and Structural Discrimination of Proline Racemase and Hydroxyproline-2-Epimerase from Nosocomial and Bacterial Pathogens
The first eukaryotic proline racemase (PRAC), isolated from the human Trypanosoma cruzi pathogen, is a validated therapeutic target against Chagas' disease. This essential enzyme is implicated in parasite life cycle and infectivity and its ability to trigger host B-cell nonspecific hypergammaglobulinemia contributes to parasite evasion and persistence. Using previously identified PRAC signature...
متن کاملPurification and Properties of Inducible Hydroxyproline 2-epimerase from Pseudomonas.
In the pathway of hydrosyproline metabolism by an inducible strain of Pseudomonas (1)) an initial reaction involves the reversible epimerization of hydroxy-L-proline to allohydroxy-n-proline by racemization at carbon 2. In subsequent steps of this sequence, allohydrosy-n-proline is oxidized to Al-pyrrolinelhydroxy2carboxylate (2) and the latter compound is oxidized to ar-ketoglutarate through a...
متن کاملpostnatal studies of bats (pipistrellus kuhlii and miniopterus schreibersii) & histomorphology and histochemistry studies of organs and diseases of (neurergus microspilotus and n. kaiseri)
1. to determine whether difference in birth body mass influenced growth performance in pipistrellus kuhlii we studied a total of 12 captive-born neonates. bats were assigned to two body mass groups: light birth body mass (lbw: 0.89 ± 0.05, n=8) and heavy birth body mass (hbw: 1.35 ± 0.08, n=4). heavier body mass at birth was associated with rapid postnatal growth (body mass and forearm length) ...
Prevalence of proline racemase/ hydroxyproline epimerase gene in human brucella isolates in Iran
Background: Human brucellosis is a zoonotic disease caused by Brucella melitensis, Brucella abortus, and Brucella suis. Brucella causes a chronic disease, which subverts the immune defense system of their hosts. In this study, the prevalence of an important Brucella virulence determinant, PrpA, which can modulate immune response, was determined in human isolates. Methods: Polymerase chain reac...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62748-6